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Assessment of Antimicrobial Activity of Fourteen Essential Oils

Assessment of Antimicrobial Activity of Fourteen Essential Oils When Using Dilution and Diffusion Methods

"The activity of 10 essential oils and 4 essential oil blends against a Gram-positive bacterium, Staphylococcus aureus, and a yeast, Candida albicans, was compared using a dilution assay and two diffusion methods. All 14 oils were active against both microorganisms. Thyme, mountain savory, and Turkish oregano were the most active oils against S. aureus with MICs of 0.31-0.42 Ál/ml. Thyme, Turkish oregano, and mountain savory also were the most active against C. albicans with MICs of 0.31-0.42 Ál/ml. For C. albicans, thyme, Melissa, mountain savory, Turkish oregano, rosewood, E2 blend, and the T1 blend were most active in the disk diffusion assay. The same oils were active against C. albicans in the hole-plate diffusion assay except for the T1 blend. Of the methods tested, the tube dilution assay best addresses the solubility and volatility concerns of essential oils. We favor the determination of MIC using INT because of several eliminated steps. Although there were differences in the way in which some essential oils responded in the two diffusion methods, the correlations between the disk and hole-plate diffusion methods were high and yielded comparable results. However, results from the dilution assay were weakly correlated with agar diffusion results. Diffusion assays are useful as a qualitative assessment of biological activity of essential oils but are not appropriate in assessing quantitative effects."

Full Article:
http://www.informaworld.com/smpp/content~db=all~content=a737685290?words=candida*|albicans



Keywords: Candida albicans diffusion methods dilution assay essential oils Staphylococcus aureus

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